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J Signal Transduct J Signal Transduct JST Journal of Signal Transduction 2090-1739 2090-1747 Hindawi Publishing Corporation PMC3100592 PMC3100592 3100592 21637351 21637351 10.1155/2010/123126 Review Article How a Mycoparasite Employs G-Protein Signaling: Using the Example of Trichoderma Omann Markus * Zeilinger Susanne Research Area of Gene Technology and Applied Biochemistry, Working Group Molecular Biochemistry of Fungi, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria *Markus Omann: omann@mail.tuwien.ac.at

Academic Editor: Terry Hebert

 2010 8 9 2010 2010 123126 20 5 2010 6 7 2010 20 7 2010 Copyright © 2010 M. Omann and S. Zeilinger. 2010 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Mycoparasitic Trichoderma spp. act as potent biocontrol agents against a number of plant pathogenic fungi, whereupon the mycoparasitic attack includes host recognition followed by infection structure formation and secretion of lytic enzymes and antifungal metabolites leading to the host's death. Host-derived signals are suggested to be recognized by receptors located on the mycoparasite's cell surface eliciting an internal signal transduction cascade which results in the transcription of mycoparasitism-relevant genes. Heterotrimeric G proteins of fungi transmit signals originating from G-protein-coupled receptors mainly to the cAMP and the MAP kinase pathways resulting in regulation of downstream effectors. Components of the G-protein signaling machinery such as Gα subunits and G-protein-coupled receptors were recently shown to play crucial roles in Trichoderma mycoparasitism as they govern processes such as the production of extracellular cell wall lytic enzymes, the secretion of antifungal metabolites, and the formation of infection structures.

 1. Introduction

All living organisms are confronted with a plethora of different stimuli due to exposure to the environment. Recognition of these stimuli and appropriate cellular responses like induction of gene transcription and protein phosphorylation are crucial for survival. Sensed at the cells surface these signals are mediated to intracellular elicitors by transmembrane signaling pathways. Fungi in particular emit sex-specific pheromones to attract potential mating partners of the opposite mating type. Surviving of pathogenic and mycoparasitic fungi depends on host-derived signals allowing them to recognize their hosts [1, 2].

For more than 70 years, species of the filamentous fungus Trichoderma have been known to be able to attack and metabolize plant pathogenic fungi, and therefore they are used as biocontrol agents [3]. Currently Trichoderma-based biological pesticides (e.g., SoilGard, Trichodex) are applied against a variety of plant pathogenic fungi like Rhizoctonia solani, Botrytis cinerea, Sclerotium rolfsii, Sclerotinia sclerotiorum, and Fusarium spp. [4–6].

Biocontrol is defined as a number of different mechanisms working synergistically to achieve disease control revealing complex interactions between biological control agents, plant pathogen, and plant [7]. These mechanisms could be either indirect like competition for nutrients and space, antibiosis and stimulation of plant-defense mechanisms, or direct like mycoparasitism [2].

Trichoderma spp. exhibit the ability to survive under unfavorable conditions predominating in ecological niches like salt marshes. Strains used in biological control should stand a wide range of temperatures, salinity, low moisture and show resistance to fungicides and chemicals used in soil treatment. These characteristics, together with their ability to produce highly efficient siderophores which chelate iron resulting in growth inhibition of other fungi make Trichoderma potent competitors [8–10]. For stimulation of plant-defense mechanisms Trichoderma produces proteins and low-molecular-weight compounds which prevent the plant from further infections [11]. Furthermore, Trichoderma secretes diverse secondary metabolites like pyrones, peptaibols, and terpenes, which can inhibit growth of plant pathogenic fungi [6].

Fungal mycoparasitism, the direct attack of one fungus on another, implies different processes occurring consecutively. These processes include recognition of the host, formation of morphological changes such as coiling around the host's hyphae and development of appressorium-like structures and subsequent penetration and killing of the host [2, 12, 13]. For penetration of the host's cell wall Trichoderma produces hydrolytic enzymes like chitinases, glucanases, and proteases [14]. To some extent production of these enzymes is already induced prior to physical contact with the host due to inducing diffusible host-derived factors [15, 16]. In addition, complementary molecules present at the surface of both the host and the mycoparasite can mediate physical contact [17]. The plant pathogen R. solani was shown to possess glycoproteins (lectins) on its surface which are able to agglutinate carbohydrate moieties present on Trichoderma hyphae [12] and thus trigger coiling of the mycoparasite around the host hyphae [4, 18].

For the activation of the mycoparasitic response, a model of different signaling pathways responding to multiple signals from the host can be assumed. This is based on findings that on the one hand lectins induce morphological changes like coiling around the host hyphae and appressorium development in Trichoderma even though they are ineffective inducers of the chitinolytic enzyme system. On the other hand cell wall degradation products are powerful inducers of chitinase production whereas they do not efficiently induce coiling. Furthermore lectins induce coiling only upon physical contact while parts of the chitinolytic enzyme system are already induced before direct contact between Trichoderma and its host.

Receptor molecules, located within the mycoparasite's cell membrane, are supposed to be the linkage between these host-derived signals and intracellular signalling pathways of Trichoderma resulting in, for example, the activation of mycoparasitism-relevant genes. Recently, examination of these intracellular signaling pathways of Trichoderma began and revealed that G-protein signaling plays an important role in mycoparasitism [19–23].

 2. G-Protein Signaling 2.1. Components of G-Protein Signaling in Fungi

Classical G-proteins are heterotrimers composed of three subunits termed Gα, Gβ, and Gγ, which are highly conserved from fungi to humans. In fungi, heterotrimeric G proteins play essential roles in sexual and pathogenic development, in secondary metabolism, in pheromone signaling cascades and processes determining fungal virulence [1, 24].

Fungal Gα subunits can be divided into three major subgroups according to a phylogenetic tree generated by multiple alignment of fungal G-protein sequences. Subgroup I Gα proteins are homologs of the mammalian Gα i subunit as both contain a consensus sequence for myristoylation (MGXXXS) at the N-terminus [25] and a site for ADP-ribosylation by pertussis toxin (CAAX) at the C-terminus [26]. Gα proteins of subgroup I lower the intracellular cAMP level by inhibiting adenylyl cyclase [27]. Among subgroup II members, protein sequences are not as well conserved as of members of groups I or III [28]. Their functions are less obvious, and their direct effectors still remain to be identified [24]. Members of subgroup III posses a myristoylation site at the N-terminus and positively influence the intracellular cAMP level. In analogy to the mammalian Gα s family, members of subgroup III have been designated as adenylyl cyclase stimulating fungal Gα s subunits [1].

Most fungal species possess one representative of each Gα subgroup. However, screening the whole genome of Saccharomyces cerevisiae revealed that GPA1 and GPA2 are the only two Gα subunit-encoding genes in this organism and they cannot be placed unambiguously into one of the three subgroups described above. GPA1 shows sequence relationship to subgroup I but lacks the consensus site for pertussis toxin-dependent ribosylation [1, 29].

By screening the genomes of Ustilago maydis and Aspergillus oryzae, a fourth Gα subunit has been identified in these fungi. Both, Gpa4 of U. maydis and GaoC of A. oryzae, exhibit some unusual features and therefore do not belong to one of the three subgroups described above. Phylogenetic analysis indicated that Gpa4 and GaoC are distinct thus excluding the presence of a conserved fourth class of Gα subunits in fungi [30, 31].

Fungal Gβ-encoding genes were shown to affect the sexual and asexual life cycle of these organisms [32]. In addition, examination of Gβ in the model filamentous fungus Neurospora crassa suggested that this subunit is also essential for the complex formation and stability of Gα and Gγ [33]. Gγ subunits form a large family of small proteins from which the majority of filamentous fungi possess only a single conserved member [32]. Gγ deletion in N. crassa for instance led to the same phenotype as deletion of the Gβ subunit such as increased conidiation, female-sterility, and decreased intracellular cAMP levels, and in addition an altered concentration of the three Gα proteins [33].

In fungi, G-protein signaling pathways elicit cellular responses like mating, cell division, growth, morphogenesis, and pathogenic development [34] but up to now only little information is available on the characteristics and functions of fungal G-protein-coupled receptors (GPCRs). Numerous fungal genomes are sequenced nowadays and comparative genomics resulted in the classification of fungal GPCRs intofor example, nine classes [31]: classes I and II include pheromone receptors related to S. cerevisiae Ste2p and Ste3p receptors; classes III and V consist of putative carbon source and cAMP sensors; class IV contains Schizosaccharomyces pombe Stm1p-like nitrogen sensors; class VI comprises a unique class in filamentous fungi representing GPCRs with an RGS domain in the cytoplasmatic moiety of the protein; members of classes VII and VIII share similarities with the rat growth hormone releasing factor (class VII) and the steroid receptor mPR (class VIII); and class IX contains fungal opsins similar to the bacterial retinal-binding rhodopsin with the well-characterized representatives NOP-1 and ORP-2 of N. crassa [35]. Subsequent to the release of the genome of the rice blast fungus Magnaporthe grisea, a large novel class of fungal GPCRs related to PTH11, a receptor required for the development of the appressorium, was defined [36, 37] and recently Zheng et al. [38] reported on the identification of three novel classes, each of which comprises one member in the plant pathogen Verticillium spp. with high sequence similarity to GPCRs of higher eukaryotes.

 2.2. Regulation of G-Protein Signaling in Fungi

Activation/deactivation and the intensity of G-protein signaling are regulated by interactions of the Gα subunit with GPCRs, Gβγ subunits, GTPase-activating, and multiple other proteins [39, 40].

GTPase-activating proteins, such as RGS (regulator of G-protein signaling) proteins, act to accelerate hydrolysis of GTP to GDP on Gα subunits and thereby terminate the transduced signal [39, 41]. While in S. cerevisiae the RGS protein Sst2 was found to control mating responses by promoting the hydrolysis of GTP on the Gα subunit Gpa1 via binding to the Ste2 pheromone receptor [42], Rgs2, the second RGS protein of yeast, negatively regulates the Gpa2 Gα subunit and glucose signaling via the Gpr1 GPCR [43]. The filamentous model fungus Aspergillus nidulans contains four RGS proteins (in addition to the RGS domain-containing GPCR GprK; [31]) among which FlbA and RgsA were shown to negatively regulate the subgroup I and III Gα subunits FadA and GanB, respectively [44, 45]. In pathogenic fungi such as Cryphonectria parasitica, M. grisea, Cryptococcus neoformans, and Metarhizium anisopliae, RGS proteins were described to regulate Gα-mediated signaling of fungal virulence [46–50].

Modulation of the activity of Gβγ subunits can be achieved by proteins belonging to the family of phosducins or phosducin-like proteins. Reports on the function of these proteins in filamentous fungi are rare. In A. nidulans and the chestnut blight fungus C. parasitica, the phosducin-like proteins PhnA and BDM-1, respectively, were shown to be necessary for Gβ function [51, 52]. In addition, BDM-1 was recently reported to be a phosphoprotein and it was shown to play a positive role in regulation of virulence [53].

In addition to regulatory proteins influencing G-protein activity, mechanisms directly regulating the activation and stability of GPCRs exist. Although, there are not yet any reports on their mode of action in filamentous fungi, regulation of the Ste2 and Ste3 pheromone receptors has been studied in detail in the model organism S. cerevisiae. GPCR signaling was shown to be regulated by ligand-triggered Ste2 receptor oligomerization and phosphorylation resulting in receptor desentization, endocytosis, and internalization [54–56]. Like Ste2, Ste3 can be recycled via ligand dependent manner [57]. In addition, the Afr1 protein was found to prevent G-protein activation via the Ste2 receptor independent of receptor phosphorylation and endocytosis [58] whereas the Asg7 protein inhibits signaling by Gβγ via a concerted action with the Ste3 pheromone receptor [59].

 3. The Role of G-Protein Signaling in <italic>Trichoderma</italic> Mycoparasitism

Comparable to fungal pathogens which attack plant, animal or human hosts, mycoparasites are pathogenic to other fungi. The mycoparasitic attack involves similar processes as those described for other pathogenic fungi such as infection-related morphogenesis, the production of hydrolytic enzymes involved in host invasion, and mycotoxin synthesis.

In plant pathogenic fungi, subgroup I and III Gα proteins and the cAMP pathway were repeatedly shown to play an essential role in regulating virulence-associated processes such as filamentation and appressorium formation. Similarly, G-protein signaling also governs pathogenesis and the production of virulence factors in various fungal human pathogens such as C. neoformans and Aspergillus fumigatus (reviewed in [24]).

Investigating different Trichoderma spp. for G-protein signaling compounds revealed that they have members of fungal Gα subgroups I, II, and III (Figure 1). Rocha-Ramírez et al. [19] silenced and overexpressed tga1, encoding the subgroup I Gα subunit in T. atroviride strain IMI 206040. Silencing of tga1 led to intense sporulation and slowly growing colonies whereas overexpression had the opposite effect by promoting vegetative proliferation and increased coiling, a morphological change associated with the mycoparasitic host attack. In direct plate confrontation assays with R. solani as the host fungus, the transformed lines overexpressing tga1 showed an impressive increase in the capacity of the fungus to overgrow and parasitize the host compared to the parental strain. On the other hand, lines blocked in the production of Tga1 were unable to overgrow the host [19].

A more profound functional characterization of Tga1 was performed by Reithner et al. [21], who extended the involvement of this G-protein α subunit to the production of antifungal metabolites and the formation of extracellular chitinases both processes relevant for the mycoparasitic host attack. tga1 knockout mutants showed strongly reduced extracellular chitinase activities and a decreased transcription of the chitinase-encoding genes nag1 (N-acetyl-glucosaminidase-encoding) and ech42 (endochitinase 42-encoding). Investigation of the antifungal activity of the Δtga1 mutant revealed reduced amounts of the major antifungal metabolite of T. atroviride, 6-pentyl-α-pyrone [21], while elevated amounts of peptaibols, peptides with antibiotic activity, could be detected [60]. These results indicate contrasting functions of Tga1 in regulating the biosynthesis of different antifungal metabolites. An elevated internal steady-state cAMP level in the Δtga1 mutants compared to the parental strain confirmed that Tga1 represents a member of the adenylyl cylcase inhibiting subgroup I of fungal Gα subunits [21].

Contrary to T. atroviride Tga1, its homologue TgaA does not influence growth or conidiation in T. virens, another mycoparasitic Trichoderma species. In antagonistic assays, when Trichoderma is confronted with a host fungus in a dual plate culture, T. virens ΔtgaA mutants showed a host-specific behavior as they could hardly colonise sclerotia of the plant pathogenic fungus S. rolfsii whereas they were fully pathogenic against another plant pathogen, R. solani. T. virens ΔtgaB mutants missing the subgroup II Gα protein revealed unaltered growth, sporulation, and mycoparasitism of R. solani and sclerotia of S. sclerotiorum [20].

Functional characterization of the subgroup III Gα protein Tga3 of T. atroviride revealed its involvement in regulating vegetative growth and conidiation. Δtga3 knockout mutants exhibited significantly reduced intracellular cAMP levels compared to the parental strain [22]. Accordingly, examination of a gna3QL mutant of the only weakly mycoparasitic species T. reesei, carrying a constitutively activated allele of the subgroup III Gα protein-encoding tga3 homologue gna3, revealed a severe increase in intracellular cAMP levels [23, 61]. This confirmed the stimulatory role of the subgroup III Gα proteins Tga3 and Gna3 on the activity of adenylyl cyclase.

Analysis of the mycoparasitic activity of T. atroviride Δtga3 mutants in antagonistic plate assays revealed that they were completely avirulent, that is, they lost the ability to attack and lyse host fungi [22]. Microscopic characterization showed that the mutants were unable to form mycoparasitism-related infection structures, like attachment to and coiling around the host hyphae. Interestingly, addition of 5 mM exogenous cAMP to the confrontation assays led to a restoration of infection structure formation. When analyzing the production of cell wall lytic enzymes in Δtga3 knockout mutants, it turned out that Tga3 is also involved in regulating this mycoparasitism-relevant process. The mutants exhibited reduced levels of extracellularly secreted chitinases compared to the parental strain although they showed elevated transcription of the chitinase-encoding genes nag1 and ech42. Further experiments revealed that chitinolytic enzymes are retained inside the cell suggesting an influence of Tga3 on chitinase gene transcription and secretion [22]. In gna3QL mutants of T. reesei elevated levels of different extracellular enzymes like endochitinase, N-acetyl-glucosaminidase, β-1,3-glucanase, lipase, and phosphatase were found [23]. In addition, the gna3QL mutants exhibited a significantly increased transcript abundance of the major cellulase-encoding gene cbh1 compared to the parental strain when the fungus was cultivated in the presence of light [61]. The authors attribute this raise in enzyme production to the elevated intracellular cAMP levels caused by the constitutively activated Gna3 protein.

In addition to regulating infection structure formation and the production of cell-wall-degrading enzymes such as chitinases, T. atroviride Tga3 was also found to be required for the production of antifungal metabolites [22]. While there is a clear correlation between sporulation of the fungus and the secretion of antifungal metabolites in the T. atroviride parental strain, Δtga3 mutants were fully impaired in the production of peptaibols although they exhibited a hypersporulating phenotype [62].

Interestingly, the Tmk1 MAP kinase was found to regulate the expression of chitinase-encoding genes in T. atroviride in a way similar to the Tga3 Gα protein [63]. This suggests that a MAPK cascade involving Tmk1 acts downstream of Tga3 in governing chitinase production.

The crucial roles of the subgroup I and III Gα proteins in regulating mycoparasitism-relevant processes implicates that identifying and clarifying the role of the corresponding G-protein-coupled receptors will be a fundamental step toward understanding the processes of host recognition and activation of the attack of phytopathogenic host fungi by mycoparasitic Trichoderma species.

Recently, analysis of GPCRs in Trichoderma started and resulted in the in silico identification of more than 50 such receptors in the genome of both T. reesei [64] as well as the mycoparasitic species T. atroviride and T. virens (S. Zeilinger, M. Omann, unpublished). Based on this analysis, four GPCR-encoding genes from the mycoparasite T. atroviride were isolated and further characterized. The obtained results showed that at least the Gpr1 receptor, grouping to the class of cAMP receptor-like (CRL) proteins (class V of fungal GPCRs), plays a major role during vegetative growth and conidiation in T. atroviride [64].

Furthermore, mutants bearing a gpr1 gene whose expression is silenced by an RNAi approach, showed a complete loss of mycoparasitism accompanied by a failure to attach to and coil around host hyphae. Interestingly, this defect in host recognition and infection structure formation could be restored by addition of exogenous cAMP [65]—similar to what was found for T. atroviride Δtga3 mutants [22]. These results suggest that Gpr1 regulates infection structure formation via the cAMP-pathway by signaling via the Tga3 Gα protein.

 4. Conclusions

Mycoparasitism comprises the interaction between two fungi involving an elaborate cross-talk of the host and the pathogen. During recent years, an increasing number of studies on the signaling pathways participating in this interaction have been performed and revealed high conservation of the investigated compounds from mycoparasitic Trichoderma to homologous proteins from other fungi.

Accordingly, signaling pathways employing, for example, Gα subunits of heterotrimeric G proteins, mitogen-activated protein kinases, adenylyl cyclases, and G-protein-coupled receptors have been shown to be important for virulence in fungi being pathogenic to plants animals/humans, as well as mycoparasites.

In mycoparasitic Trichoderma species, both subgroup I and subgroup III Gα proteins were shown to govern mycoparasitism-relevant processes such as the production of cell wall lytic enzymes and antifungal metabolites and the formation of infection structures. Both subgroup I and III Gα proteins of T. atroviride signal—at least partially—via the cAMP pathway as Tga1 was proven to negatively influence the activity of adenylate cyclase whereas Tga3 stimulated its activity (Figure 2).

The essential role of G-protein signaling in activation of the mycoparasitic response of Trichoderma was further supported by the functional characterization of T. atroviride Gpr1. Similar to the Tga3 Gα subunit, the Gpr1 G-protein-coupled receptor seems to be involved in recognizing host-derived signals and transducing them via the cAMP pathway. Gpr1, therefore, is the first GPCR from a mycoparasitic fungus which was functionally characterized and the first 7-transmembrane receptor belonging to the CRL class of fungal GPCRs for which pathogenicity-related functions could be shown.

Although during recent years there has been considerable progress in elucidating G-protein-mediated signaling in pathogenic fungi, there are still many unsolved questions especially concerning host recognition and regulation of signaling events. The elucidation of these processes will for sure be highly beneficial not only for developing mechanisms and substances for combating these pathogens, but also for a better understanding of the molecular processes underlying fungal mycoparasitism.

 Acknowledgements

The described work performed by the authors on T. atroviride signal transduction was supported by Grants from the Fonds zur Förderung Wissenschaftlicher Forschung (FWF P15483 and P18109). S Zeilinger is recipient of an Elise-Richter fellowship (V139-B20) funded by FWF.

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Relationship of fungal Gα amino acid sequences of subgroups I and III whose members Tga1 and Tga3 were shown to be essential for mycoparasitism of T. atroviride. In addition, the fourth Gα subunit only present in U. maydis (Gpa4) and A. oryzae (GaoC) were included and are indicated as subgroup IV. The tree was generated using Neighbor-Joining algorithm subsequent to CLUSTALX alignment. The fourth Gα subunit of U. maydis Gpa4 has been defined as outgroup. Abbreviations used: Ao: Aspergillus oryzae; Mg: Magnaporthe grisea; Nc: Neurospora crassa; Ta: Trichoderma atroviride; Tr: Trichoderma reesei; Tv: Trichoderma virens; Um: Ustilago maydis.

Schematic illustration of G-protein signaling in Trichoderma atroviride. GPCR: G protein-coupled receptor; Tga1, Tga3: subgroup I and III G-protein α subunits; Tmk1: MAP kinase.